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KMID : 0357319960310050591
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Journal of the Korean Society for Microbiology
1996 Volume.31 No. 5 p.591 ~ p.598
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Indentification of Adenovirus 40, 41 in Feces from Young Children with Diarrhea by Polymerase Chain Reaction
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Abstract
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Adenovirus is the second leading cause of acute gastroenteritis. As the children with diarrhea by acute adenoviral gastroentertis usually require hospitalization, rapid diagnosis is necessary for proper management. Adenovirus infection can be
diagnosed
conventionally by the direct electron microscopic observation of the virus particle, isolation of the virus by cell culture, or enzyme immuno assay. Recently, polymerase chain reaction is applied for the detection of adenovirus. In this study, we
investigated stools collected from 100 children hospitalized for diarrhea in Ewha Womans University Hospital from the September of 1995 to the July of 1996 for adenovirus by polymerase chain reaction.
Two oligonucleotide primers, desiginated as hexAA1885 and hexAA1913, were used for the detection of enteric adenovirus type 40 and 41 in PCR. After amplification of 35 cycles, 300 bp framents were detected on 1.8% Sea Kem GT agar gel in 14
samples.
The
stool samples were further analyzed by electron microscopy and immunoblotting. In electron microscopy, icosahedral particles of about 75nm were observed in 7 of the 14 samples. The 14 samples. The 14 samples were collected from children whose
mean
age
was 1.4 years and the sex ratio was 10/4 (M/F). The seasonal variation showed acute gastroenteritis by adenovirus infection was frequent in March to May and in September to October in Korea.
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KEYWORD
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